Effects of Trifolium pratense

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• 作者：Dé ; cio Luis Alves ; Sô ; nia Maria Rolim Rosa Lima ; Cé ; lia Regina da Silva ; Maria Antonieta Longo Galvã ; o ; Alberto Shanaider ; Roberto Adelino de Almeida Prado ; Tsutomu Aoki
• 关键词：Estrogen receptors ; Ki67 antigen ; Trifolium pratense ; Cimicifuga racemosa ; Morphology ; Endometrium
• 刊名：Maturitas
• 出版年：2008
• 出版时间：20 December 2008
• 年：2008
• 卷：61
• 期：4
• 页码：364-370
• 全文大小：1176 K

文摘

Objective

The aim of this study was to evaluate the effects of Trifolium pratense and Cimicifuga racemosa upon the endometrium of castrated female Wistar rats, comparing these results with a placebo and estradiol valerate.

Methods

Thirty-two adult castrated female Wistar rats were divided into four groups (eight rats per group) that receiving either tap water, estradiol valerate, isoflavones from T. pratense or deoxyactein from C. racemosa daily. The doses used were equivalent to normal doses used in humans. The results were analyzed by endometrial histology and the expression of α-estrogen receptor and protein Ki67. Both α-receptor and Ki67 expressions were determined by immunohistochemical and morphometric analysis.

Results

Endometrium histology stayed atrophic with both herbal extracts, but T. pratense supplementation increased the expression of α-estrogen receptors when compared to the placebo group, without protein Ki67 expression enhancement. Both herbal extracts presented a lower Ki67 expression when compared to the placebo group.

Conclusion

T. pratense presented α-estrogen receptor stimulation in the endometrium without increasing cell proliferation. Both herbal extracts reduced endometrial proliferation in comparison to the placebo group.