Th1 biased response to a novel Porphyromonas gingivalis protein aggravates bone resorption caused by this oral pathogen

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• 作者：Onir Leshem ; Suely S. Kashino ; Reginaldo B. Gonç ; alves ; Noriyuki Suzuki ; Masao Onodera ; Akira Fujimura ; Hajime Sasaki ; Philip Stashenko ; Antonio Campos-Neto
• 关键词：Prophyromonas gingivalis ; Th1 response ; Bone resorption
• 刊名：Microbes and Infection
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：10
• 期：6
• 页码：664-672
• 全文大小：723 K

文摘

In previous studies we showed that biasing the immune response to Porphyromonas gingivalis antigens to the Th1 phenotype increases inflammatory bone resorption caused by this organism. Using a T cell screening strategy we identified eight P. gingivalis genes coding for proteins that appear to be involved in T-helper cell responses. In the present study, we characterized the protein encoded by the PG_1841 gene and evaluated its relevance in the bone resorption caused by P. gingivalis because subcutaneous infection of mice with this organism resulted in the induction of Th1 biased response to the recombinant PG1841 antigen molecule. Using an immunization regime that strongly biases toward the Th1 phenotype followed by challenge with P. gingivalis in dental pulp tissue, we demonstrate that mice pre-immunized with rPG1841 developed severe bone loss compared with control immunized mice. Pre-immunization of mice with the antigen using a Th2 biasing regime resulted in no exacerbation of the disease. These results support the notion that selected antigens of P. gingivalis are involved in a biased Th1 host response that leads to the severe bone loss caused by this oral pathogen.