Ten biodiesel types from various raw materials were studied in composition free steroids.
Contents of steroids present in biodiesel determined by GC-FID showed great variability among them.
Results obtained by GC–MS were used in identification of steroids quantified.
Types of steroids present in biodiesel and its own peculiar distribution showed that some steroids were exclusive.
Composition of steroids in this study allows the identification of different types of biodiesel in a blend them.