- 作者：Ma¨ªra Ribeiro de Souza ; Carmen Aparecida de Paula ; Michelle Luciane Pereira de Resende ; Andrea Grabe-Guimar?es ; Jos¨¦ Dias de Souza Filho ; D¨ºnia Antunes Sa¨²de-Guimar?es
- 关键词：Lychnophora trichocarpha ; Flavones ; Sesquiterpene lactones ; Hyperuricemia ; Xanthine oxidase ; Gout
- 刊名：Journal of Ethnopharmacology
- 出版年：2012
- 出版时间：1 August, 2012
- 年：2012
- 卷：142
- 期：3
- 页码：845-850
- 全文大小：455 K
Ethnopharmacological relevance
The ethanolic extract of Lychnophora trichocarpha Spreng. is used in Brazilian folk medicine to treat bruise, pain and inflammatory diseases.Aim of the study
The present study aimed at investigating whether ethanolic extract of L. trichocarpha, its ethyl acetate fraction and its main bioactive compounds could be useful to treat gouty arthritis by countering hyperuricemia and inflammation.
Materials and methods
L. trichocarpha ethanolic extract (LTE), ethyl acetate fraction from ethanolic extract (LTA) and isolated compounds were evaluated for urate-lowering activity and liver xanthine oxidase (XOD) inhibition in oxonate-induced hyperuricemic mice. Anti-inflammatory activity in monosodium urate crystal-induced paw oedema, an experimental model of gouty arthritis, was also investigated.
Results
Crude ethanolic extract and its ethyl acetate fraction showed significant urate-lowering effects. LTE was also able to significantly inhibit liver xantine oxidase (XOD) activity in vivo at the dose of 250 mg/kg. Luteolin, apigenin, lupeol, lychnopholide and eremantholide C showed the anti-hyperuricemic activities among tested compounds. Apigenin also showed XOD inhibitory activity in vivo. Luteolin, lychnopholide, lupeol and eremantholide C, in turn, did not shown significant inhibitory activity towards this enzyme, indicating that this mechanism is not likely to be involved in urate-lowering effects of those compounds. LTE, LTA, lupeol, ¦Â-sitosterol, lychnopholide, eremantholide, luteolin and apigenin were also found to inhibit monosodium urate crystals-induced paw oedema in mice.
Conclusions
Ethanolic extract of Lychnophora trichocarpha and some of its bioactive compounds may be promising agents for the treatment of gouty arthritis since they possesses both anti-hiperuricemic and anti-inflammatory properties.