SPE method was standardized for isolation of aspartame and neotame in flavoured milk.
Pasteurization resulted in ∼40% and 8% loss of aspartame and neotame, respectively.
Storage (4–7 °C/7 days) caused ∼15% and 4% loss of aspartame and neotame respectively.
Sterilization caused complete loss of aspartame but ∼50% of neotame remained intact.
During storage (30 °C/60 days) neotame content decreased from 50.36% to 8.67%.