Characterization of Pseudoalteromonas citrea and P. nigrifaciens Isolated from Different Ecological Habitats Based on REP-PCR Genomic Fingerprints

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• 作者：Elena P. Ivanova ; Glavur R. Matte ; Maria H. Matte ; Tom Coenye ; Anwarul Huq ; Rita R. Colwell
• 关键词：Pseudoalteromonas ; biogeography ; REP ; ERIC ; BOX-PCR
• 刊名：Systematic and Applied Microbiology
• 出版年：2002
• 出版时间：2002
• 年：2002
• 卷：25
• 期：2
• 页码：275-283
• 全文大小：1480 K

文摘

DNA primers corresponding to conserved repetitive interspersed genomic motifs and PCR were used to show that REP, ERIC and BOX-like DNA sequences are present in marine, oxidative, Gram-negative Pseudoalteromonas strains. REP, ERIC and BOX-PCR were used for rapid molecular characterization of both the type species of the genus and environmental strains isolated from samples collected in different geographical areas. PCR-generated genomic fingerprint patterns were found to be both complex and strain specific. Analysis of the genotypic structure of phenotypically diverse P. citrea revealed a geographic clustering of Far Eastern brown-pigmented, agar-digesting strains of this species. Marine isolates of P. nigrifaciens with 67–70 % DNA relatedness generated genomic patterns different from those of the type strain and formed a separate cluster. It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion.