Stable production of recombinant chicken antibody in CHO-K1 cell line

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• 作者：Toshi Shimamoto ; Nahoko Nishibori ; Masayoshi Aosasa ; Hiroyuki Horiuchi ; Shuichi Furusawa and Haruo Matsuda
• 关键词：Recombinant antibody ; Chicken ; IgY ; CHO
• 刊名：Biologicals
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：33
• 期：3
• 页码：169-174
• 全文大小：199 K

文摘

When compared with mammalian IgG, chicken IgY is advantageous in terms of cross-reactivity. In this study, two plasmids were constructed for expression of recombinant chicken IgY derived from a chicken hybridoma. The first was for expression of the light (L) chain, and the other was for the heavy (H) chain with a histidine (His) tag at the carboxy-terminal. After transfection of recombinant chicken IgY gene into Chinese hamster ovary cells, a transfectant designated HF33 that secreted the specific antibody was selected. HF33 cells produced recombinant IgY with His tag at 10–15 μg/10⁶ cells/24 h. On Western blotting analysis, the recombinant IgY was detected as one band for the H chain and two bands for the L chain. The recombinant IgY was successfully purified in a one-step procedure using a nickel-affinity resin. These results indicate that the present recombinant chicken IgY is useful for further applications.