Characterization and expression analysis of the chicken interleukin-11 receptor alpha chain
详细信息 查看全文
- 作者：Kawashima ; Tsuyoshi ; Hojyo ; Shintaro ; Nishimichi ; Norihisa ; Sato ; Masaharu ; Aosasa ; Masayoshi ; et. al.
- 关键词：Chickens ; Cytokine ; Interleukin-11 receptor ; Immunoglobulin c2-type domain ; Fibronectin (FN) type-III domain ; WSXWS motif ; Expression ; Ovary ; Chicken blastodermal cells (CBCs) ; IL-11 ; Interleukin-11 ; chIL-11R ; chicken IL-11 receptor ; Ig-c2-type domain ; im
- 刊名：Developmental and Comparative Immunology
- 出版年：2005
- 出版时间：2005
- 年：2005
- 卷：29
- 期：4
- 页码：349-359
- 全文大小：582 K
文摘
Interleukin-11 (IL-11) is a multifunctional cytokine involved in various pathways in blood cells, their precursors and many other cell types in vitro and in vivo. The effects of IL-11 are largely mediated by the IL-11 receptor α-chain (IL-11Rα). In this study, a putative cDNA sequence encoding the 414 amino acid propeptide of chicken IL-11R (chIL-11R) was identified. The predicted 414 amino acid sequence showed 42–43 % sequence identity with mammalian homologues. In a domain search of the molecule, two fibronectin (FN) type-III domains were identified in the C- terminal portion. On comparison with mammalian IL-11R, 4 conserved cysteine residues and a WSXWS motif were observed within the FN type-III domains. Expression analysis revealed that chIL-11Rα is strongly expressed in brain, heart, lung, liver, glandular stomach, kidney, the immature testis, ovary and chicken blastodermal cells (CBCs) after 1-day-cultivation. These findings strongly indicate that the identified chicken cDNA sequence encodes chIL-11R α-chain homologue.