Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A_2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom

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• 作者：L.M. Resende^a ; J.R. Almeida^b ; ^f ; ^{rafael.dealmeida@ikiam.edu.ec} ; R. Schezaro-Ramos^c ; R.C.O. Collaç ; o^c ; L.R. Simioni^c ; D. Ramí ; rez^d ; W. Gonzá ; lez^d ; ^f ; A.M. Soares^e ; ^f ; L.A. Calderon^e ; ^f ; S. Marangoni^a ; S.L. da Silva^b ; ^f
• 关键词：Acidic phospholipase A2 ; Snake venom ; Agkistrodon piscivorus leucostoma ; Pharmacological activity
• 刊名：Toxicon
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：127
• 期：Complete
• 页码：22-36
• 全文大小：3374 K
• 卷排序：127

文摘

AplTx-I is an acidic and monomeric phospholipase A2, isolated from Agkistrodon piscivorus leucostoma venom. AplTx-I has high catalytic activity on synthetic and non-micellar substrate. The amino acid sequence of AplTx-I was identified by mass spectrometry. In vitro and in vivo, AplTx-I presented low-level, or lack of, toxicity to mammals and bacteria. AplTx-I promoted an irreversible and selective neuromuscular blockade in biventer cervicis preparations.