Multi-parametric imaging of cell heterogeneity in apoptosis analysis

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• 作者：Ivan A. Vorobjev^a ; ^b ; ^c ; ^{vorob@libro.genebee.msu.ru} ; Natasha S. Barteneva^d ; ^e
• 关键词：7-AAD ; 7-aminoactinomycin D ; AB ; antibody ; AcrDIMs ; 3 ; 6-bis((1-alkyl-5-oxo-imidazolidin-2-yliden)imino)acridine hydrochlorides ; AIF ; apoptosis-inducing factor ; AnV ; annexin V ; AO ; acridin orange ; BRDU ; bromodeoxyuridine ; AVD ; apoptotic volume decrease ; Bax ; Bcl-2 associated X-protein ; BID ; BH3-interacting-domain death agonist ; BODIPY ; boron-dipyrromethene-based fluorescent lipid dyes ; bp ; base pair ; CAD ; caspase-activated DNAse ; CCD ; charging coupling devices ; C-FLIP ; FLICE-like inhibitory prote
• 刊名：Methods
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：105-123
• 全文大小：4483 K
• 卷排序：112

文摘

During apoptosis morphological and molecular events occur simultaneously and/or consequently. Analysis of apoptosis requires quantitation of multiparametric data and morphological analysis. Efficient analysis of apoptosis is based on combined fluorescent microscopy, flow and imaging flow cytometry. Benefits and limitations of imaging flow cytometry in apoptosis analysis are discussed. Dyes and protocols for multicolor labeling and data analysis are described.