Reference gene validation for qRT-PCR based gene expression studies in different developmental stages and under biotic stress in apple
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- 作者：Gulshan Kumara ; b ; Anil Kumar Singha ; b ; anil@ihbt.res.in" class="auth_mail" title="E-mail the corresponding author ; anils13@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：18S ; 18S ribosomal RNA ; ACT ; actin ; APRT ; adenine phosphoribosyl transferases ; CT ; cycle threshold ; CYP ; cyclophilin ; EF-1α ; elongation factor-1α ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; PP2A ; protein phosphatase 2A ; qRT-PCR ; quantitative real-time polymerase chain reaction ; RPL2 ; ribosomal protein L2 ; TUB ; β-tubulin ; UBQ ; ubiquitin
- 刊名：Scientia Horticulturae
- 出版年：2015
- 出版时间：14 December 2015
- 年：2015
- 卷：197
- 期：Complete
- 页码：597-606
- 全文大小：1098 K
文摘
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Ten putative reference genes for qRT-PCR data normalization have been identified in apple.
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Stages of bud to fruit development and samples under biotic stresses were analyzed.
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PCR efficiencies of all the genes were found to be ranging between 94% and 107.6%.
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PP2A, RPL2 and GAPDH were found to be stable reference genes across the samples.
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The 18S rRNA, β-tubulin and ubiquitin were found to be the least stable genes.