High-throughput assays of leloir-glycosyltransferase reactions: The applications of rYND1 in glycotechnology
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- 作者：Yijun Lia ; Jin Houb ; Fengshan Wanga ; c ; fswang@sdu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Juzheng Shenga ; c ; shengjuzheng@sdu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：GlcA-pNP ; 1-O-(para-Nitrophenyl)-glucuronide ; Glmu ; glucosamine-1-phosphate acetyltransferase/N-acetylglucosamine-1-phosphate uridyltransferase ; GT ; glycosyltransferases ; KfiA ; N-acetylglucosaminyltransferase from E. coli K5 ; KfoC ; chondroitin synthase from E. coli K4 strain ; NDPase ; NDP-based nucleoside-diphosphatase ; UDP-GlcNH2 ; UDP-glucosamine ; UDP-GlcNTFA ; UDP-N-trifluoroacetylated glucosamine ; PAMN ; polyamine-based anion exchange ; Pi ; inorganic phosphate ; PmHS2 ; heparosan synthase 2 from Pa
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：227
- 期：Complete
- 页码：10-18
- 全文大小：1766 K
文摘
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A soluble recombinant expression method of S. cerevisiae membrane-bond apyrase YND1.
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A rYND1-coupled universal Leloir-glycosyltransferase screening approach.
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Positive Effect of rYND1 on glycosyltransferase-catalyzed saccharide synthesis.