Peanut protein extraction conditions strongly influence yield of allergens Ara h 1 and 2 and sensitivity of immunoassays
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- 作者：Nicole E. Walczyk<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>walczyk.nicole@gmail.comsup> ; Penelope M.C. Smith<sup>bsup><sup> ; sup><sup>penny.smith@sydney.edu.ausup> ; Euan R. Tovey<sup>csup><sup> ; sup><sup>euan.tovey@sydney.edu.ausup> ; Thomas H. Roberts<sup>asup><sup> ; sup><sup>dsup><sup> ; sup><sup>thomas.roberts@sydney.edu.ausup>
- 关键词：Ara h 1 ; 2 ; 3 ; etc. ; Arachis hypogaea allergen 1 ; 2 ; 3 ; etc. ; ELISA ; enzyme-linked immunosorbent assay ; IEF ; isoelectric focusing
- 刊名：Food Chemistry
- 出版年：2017
- 出版时间：15 April 2017
- 年：2017
- 卷：221
- 期：Complete
- 页码：335-344
- 全文大小：1846 K
- 卷排序：221
文摘
Defatting peanut meal with n-hexane gave significantly more Ara h 1 and 2 than with diethyl ether. Buffer type and pH affected extraction yields and detection rates of Ara h 1 and 2 in ELISAs. Extraction yields of crude peanut protein correlated poorly with yields of Ara h 1 and Ara h 2. Western blots of allergens extracted with PBS were more sensitive than those extracted with TBS or Tris. Common extraction buffers could retain allergens in peanut meal; e.g. TBS (pH 8.5) retained Ara h 3.