The Arabidopsis thaliana MEK AtMKK6 activates the MAP kinase AtMPK13
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- 作者：Melikant ; Balá ; zs ; Giuliani ; Concetta ; Halbmayer-Watzina ; Susanne ; Limmongkon ; Apinun ; et. al.
- 关键词：HIS ; histidine ; MAP kinase ; mitogen-activated protein kinase ; MBP ; myelin basic protein ; MEK ; MAP kinase kinase ; RT-PCR ; reverse transcription polymerase chain reaction ; URA ; uracil ; Arabidopsis ; Mitogen-activated protein kinase ; MAP kinase kinase
- 刊名：FEBS Letters
- 出版年：2004
- 出版时间：October 8, 2004
- 年：2004
- 卷：576
- 期：1-2
- 页码：5-8
- 全文大小：425 K
文摘
Mitogen-activated protein (MAP) kinases mediate cellular responses to a wide variety of stimuli. Activation of a MAP kinase occurs after phosphorylation by an upstream dual-specificity protein kinase, known as a MAP kinase kinase or MEK. The Arabidopsis thaliana genome encodes 10 MEKs but few of these have been shown directly to activate any of the 20 Arabidopsis MAP kinases. We show here that functional complementation of the cell lysis phenotype of a mutant yeast strain depends on the co-expression of the Arabidopsis MEK AtMKK6 and the MAP kinase AtMPK13. The kinase activity of AtMPK13 is stimulated in the presence of AtMKK6 in yeast cells. RT-PCR analysis showed the co-expression of these two genes in diverse plant tissues. These data show that AtMKK6 can functionally activate the MAP kinase AtMPK13.