Functional analysis of CaRAP1, encoding the Repressor/activator protein 1 of Candida albicans
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- 作者：Biswas ; Kajal ; Rieger ; Klaus-Jö ; rg ; Morschhä ; user ; Joachim
- 关键词：Essential gene ; Filamentous growth ; Pseudohyphae ; RAP1 ; BSA ; bovine serum albumin ; CSM ; complete supplement medium ; ECL ; enhanced chemiluminescence ; SAP ; secreted aspartic proteinase ; UV ; ultraviolet ; W ; watt ; YCB ; yeast carbon base ; YNB ; yeast nitrogen b
- 刊名：Gene
- 出版年：2003
- 出版时间：March 27, 2003
- 年：2003
- 卷：307
- 期：Complete
- 页码：151-158
- 全文大小：379 K
文摘
The RAP1 gene (repressor/activator protein 1) encodes a transcription factor and telomere binding protein that is essential for viability in the budding yeast Saccharomyces cerevisiae. The genome sequence of the opportunistic fungal pathogen Candida albicans contains a RAP1 homologue. We generated C. albicans mutants in which both RAP1 alleles were deleted. The Δrap1 mutants grew as well as the wild-type parental strain and formed normal germ tubes and hyphae in response to a variety of inducing conditions. However, under conditions that promote budding yeast growth in the wild-type strain, the Δrap1 mutants formed both yeast and pseudohyphal cells. This phenotype was reverted upon reintroduction of a functional RAP1 copy. Our results demonstrate that RAP1 is a non-essential gene in C. albicans which is required to repress the formation of pseudohyphae under conditions favouring growth as budding yeast.