Ultradeformable archaeosomes as new topical adjuvants

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• 作者：Leticia H. Higa ; MS ; Priscila Schilrreff ; MS ; Ana Paula Perez ; PhD ; Maiara A. Iriarte ; MS ; Diana I. Roncaglia ; PhD ; Maria Jose Morilla ; PhD ; Eder L. Romero ; PhD ; ^{elromero@unq.edu.ar}
• 关键词：Vesicles ; Skin ; Topical adjuvants
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：8
• 期：8
• 页码：1319-1328
• 全文大小：753 K

文摘

Ultradeformable archaeosomes (UDA) are vesicles made of soybean phosphatidylcholine (SPC), sodium cholate (NaChol) and polar lipids from Halorubrum tebenquichense (3:1:3 wt/wt). Although ultradeformable liposomes (UDL, made of SPC and NaChol at 6:1 wt/wt) and UDA were neither captured nor caused cytotoxicity on keratinocytes, UDA was avidly captured by macrophages, their viability being reduced by 0.4-1.6 mg/mL phospholipids by 25 to 60 % . Instead, UDL were poorly captured and caused no toxicity. Balb/C mice immunized by the topical route with four doses of ovalbumin (OVA)-loaded UDA, at 75 ¦Ìg OVA/600 ¦Ìg phospholipids (125 nm mean size and -42 mV zeta potential), induced IgG titers tenfold to 100-fold higher than those immunized with OVA-loaded UDL at the same dosage. Both matrices penetrate to the same skin depth (nearly 10 ¦Ìm after 1 hour on excised human skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to its glycolipid content.

From the Clinical Editor

This work summarizes key findings related to the development of ultradeformable archaeosomes as vehicles utilized in transdermal delivery systems with improved skin penetration.