Studies on the arctiin and its interaction with DNA by spectral methods

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• 作者：Yantao Sun^a ; ^b ; ^c ; Hanqi Zhang^d ; Shuyun Bi^e ; Xiaofu Zhou^f ; Liang Wang^b ; ^{1979yanzi@163.com"" rel=""nofollow} ; Yongsheng Yan^a ;
• 关键词：Arctiin ; DNA ; Fluorescence spectrometry ; Interaction
• 刊名：Journal of Luminescence
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：131
• 期：11
• 页码：2299-2306
• 全文大小：381 K

文摘

The emission spectra of arctiin were determined under various experimental conditions. In addition, a fluorescence method was developed to obtain the binding constants and sites of the interaction between arctiin and DNA. A competitive binding experiment and melting temperature mensuration were carried out to investigate the binding mechanism of arctiin and DNA. The experimental results showed that the interaction between arctiin and DNA belongs to a groove binding mode.