Self-assembly of ATP synthase subunit c rings

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• 作者：Arechaga ; Ignacio ; Butler ; P. Jonathan G. ; Walker ; John E.
• 关键词：F-ATPase ; Subunit c ; Oligomerisation ; Analytical Ultracentrifugation ; Electron microscopy
• 刊名：FEBS Letters
• 出版年：2002
• 出版时间：March 27, 2002
• 年：2002
• 卷：515
• 期：1-3
• 页码：189-193
• 全文大小：328 K

文摘

Subunit c of the H⁺ transporting ATP synthase is an essential part of its membrane domain that participates in transmembrane proton conduction. The annular architecture of the subunit c from different species has been previously reported. However, little is known about the type of interactions that affect the formation of c-rings in the ATPase complex. Here we report that subunit c over-expressed in Escherichia coli and purified in non-ionic detergent solutions self-assembles into annular structures in the absence of other subunits of the complex. The results suggest that the ability of subunit c to form rings is determined by its primary structure.