δ-Endotoxins induce cation channels in Spodoptera frugiperda brush border membranes in suspension and in planar lipid bilayers
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- 作者：Lorence ; Argelia ; Darszon ; Alberto ; Dí ; az ; Claudia ; Lié ; vano ; Arturo ; Quintero ; Rodolfo ; et. al.
- 关键词：δ ; -Endotoxin ; Mode of action ; Ionic channel ; Bacillus thuringiensis
- 刊名：FEBS Letters
- 出版年：1995
- 出版时间：March 6, 1995
- 年：1995
- 卷：360
- 期：3
- 页码：217-222
- 全文大小：530 K
文摘
Membrane potential measurements using a fluorescent dye indicated that two specific toxins active against Spodoptera frugiperda larvae (CryIC and CryID) cause immediate permeability changes in midgut epithelial brush border membrane vesicles (BBMV). The initial response and the sustained permeability change are cationic, not very K+ selective, and occur at in vivo lethal doses (nM). The toxin response has a different ion selectivity and is more sensitive to Ba2+ than the intrinsic cation permeability of BBMV. Experiments incorporating BBMV into planar lipid bilayers (PLB) demonstrated that these vesicles contain cation channels (31, 47 and 76 pS). A 2-40 fold conductance increase was induced by nM concentrations of toxin in PLB containing BBMV. Cationic single channel transitions of 50, 106, 360 and 752 pS were resolved. Thus, Bacillus thuringiensis δ-endotoxins induce an increase in cation membrane permeability involving ion channels in BBMV-containing functional receptors.