Different cross-reactivity of human and rodent sera to Tula virus and Puumala virus

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• 作者：Erdenesaikhan Tegshduuren ; Kumiko Yoshimatsu ; Midori Taruishi ; Rika Endo ; Kenta Shimizu ; Takaaki Koma ; Shumpei P. Yasuda ; Hiroaki Kariwa ; Jiro Arikawa ; Chiaki Ishihara
• 关键词：Hantavirus ; TULV ; PUUV ; Japanese grass vole ; IFA ; ELISA ; RT-PCR ; Microtus ; Myodes ; HFRS
• 刊名：Comparative Immunology, Microbiology and Infectious Diseases
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：33
• 期：6
• 页码：e67-e73
• 全文大小：455 K

文摘

Tula virus (TULV) and Puumala virus (PUUV) are hantaviruses carried by the bank vole (Myodes glareolus) and European common vole (Microtus arvalis), respectively. PUUV is a causative agent of hemorrhagic fever with renal syndrome (HFRS), while TULV is thought to be apathogenic to humans. The N-terminal regions of the N proteins from TULV and PUUV were expressed and applied as enzyme-linked immunosorbent assay (ELISA) antigens. Colonized Japanese grass voles (Microtus montebelli) and BALB/c mice were used for experimental inoculation of the vole-borne hantaviruses TULV and PUUV. Voles and mice showed significant antibody production toward both viruses, but these antisera showed little cross-reactivity between TULV and PUUV in the immunofluorescence antibody assay and ELISA. In contrast, sera from patients with HFRS caused by PUUV exhibited high cross-reactivity against the TULV antigen, and sera from a natural rodent reservoir showed moderate cross-reactivity against the heterologous antigen, indicating that the antigenic cross-reactivity between TULV and PUUV differs in sera from rodents and humans.