Simultaneous determination of nine aristolochic acid analogues in medicinal plants and preparations by high-performance liquid chromatography

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• 作者：Jinbin Yuan ; Lihua Nie ; Deyu Zeng ; Xubiao Luo ; Fei Tang ; Li Ding ; Qian Liu ; Manli Guo ; Shouzhuo Yao
• 关键词：Aristolochic acid analogues ; HPLC ; Aristolochiaceae ; Traditional chinese medicine ; Herbal preparation
• 刊名：Talanta
• 出版年：2007
• 出版时间：15 October 2007
• 年：2007
• 卷：73
• 期：4
• 页码：644-650
• 全文大小：544 K

文摘

By optimizing the extraction, separation and analytical conditions, a simple, reliable and effective high-performance liquid chromatography method coupled with photodiode array detector (HPLC–DAD) is presented for simultaneous determination of nine aristolochic acid (AA) analogues, i.e., AA I, AA II, AA C, AA D, 7-OH AA I, aristolic acid, AL II, AL III and AL IV, in twelve medicinal herbs and two preparations. The separation was completed on a C18 column with aqueous methanol containing 0.2 % (V/V) acetic acid as mobile phase. Linearities of around two orders of magnitude were obtained with correlation coefficients exceeding 0.9950. Satisfactory intra-day and inter-day precisions were achieved with R.S.D.s less than 4.35 % , and the average recovery factors obtained were in the range of 88.4–98.8 % . The proposed method appears to be suitable for use as a tool for safety assurance and quality control for commercially available suspect samples containing aristolochic acid analogues.