Interaction of cationic carbosilane dendrimers and their complexes with siRNA with erythrocytes and red blood cell ghosts

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• 作者：Dominika Wrobel ; Katarzyna Kolanowska ; Arkadiusz Gajek ; Rafael Gomez-Ramirez ; Javier de la Mata ; El偶bieta Pedziwiatr-Werbicka ; Barbara Klajnert ; Iveta Waczulikova ; Maria Bryszewska
• 关键词：Carbosilane ; Red blood cell ghost ; Hemolysis ; Membrane fluidity ; HIV-1 ; siRNA
• 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
• 出版年：March, 2014
• 年：2014
• 卷：1838
• 期：3
• 页码：882-889
• 全文大小：1007 K

文摘

We have investigated the interactions between cationic NN16 and BDBR0011 carbosilane dendrimers with red blood cells or their cell membranes. The carbosilane dendrimers used possess 16 cationic functional groups. Both the dendrimers are made of water-stable carbon-silicon bonds, but NN16 possesses some oxygen-silicon bonds that are unstable in water. The nucleic acid used in the experiments was targeted against GAG-1 gene from the human immunodeficiency virus, HIV-1.

By binding to the outer leaflet of the membrane, carbosilane dendrimers decreased the fluidity of the hydrophilic part of the membrane but increased the fluidity of the hydrophobic interior. They induced hemolysis, but did not change the morphology of the cells. Increasing concentrations of dendrimers induced erythrocyte aggregation. Binding of short interfering ribonucleic acid (siRNA) to a dendrimer molecule decreased the availability of cationic groups and diminished their cytotoxicity. siRNA-dendrimer complexes changed neither the fluidity of biological membranes nor caused cell hemolysis. Addition of dendriplexes to red blood cell suspension induced echinocyte formation.