Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up

详细信息    查看全文

• 作者：Juan José ; Villaverde^a ; ^{juanjose.villaverde@inia.es} ; Vincent van der Vlist^b ; S&#243 ; nia A.O. Santos^a ; Thomas Haarmann^c ; Kim Langfelder^c ; Minni Pirttimaa^d ; Antti Nyyssö ; lä ; ^d ; Sirpa Jylhä ; ^d ; Tarja Tamminen^d ; Kristiina Kruus^d ; Leo de Graaff^b ; Carlos Pascoal Neto^a ; Má ; rio M.Q. Simõ ; es^e ; M.R.M. Domingues^e ; Armando J.D. Silvestre^a ; Jasmin Eidner^c ; Johanna Buchert^d
• 关键词：Lipoxygenase ; Linoleic acid ; Fatty acid hydroperoxides ; Optimization ; Scale-up
• 刊名：Chemical Engineering Journal
• 出版年：2013
• 出版时间：1 February, 2013
• 年：2013
• 卷：217
• 期：Complete
• 页码：82-90
• 全文大小：753 K

文摘

Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 ¡ãC and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0 % ) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4 % ) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100-300 g/L) leading to HPODE yields of ¡«40 % and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74 % .