Selective activation of phospholipase D2 by unsaturated fatty acid

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• 作者：Kim ; Jae Ho ; Kim ; Yong ; Lee ; Sang Do ; Lopez ; Isabel ; Arnold ; Rebecca S. ; Lambeth ; J. David ; Suh ; Pann-Ghill ; et. al.
• 关键词：Phospholipase D2 ; Oleic acid ; Phosphatidylinositol 4 ; 5-bisphosphate ; PLD ; phospholipase D ; PIP₂ ; phosphatidylinositol 4 ; 5-bisphosphate ; PC ; phosphatidylcholine ; PA ; phosphatidic acid ; ARF ; ADP-ribosylation factor ; PKC ; protein kinase C ; PE ; pho
• 刊名：FEBS Letters
• 出版年：1999
• 出版时间：July 2, 1999
• 年：1999
• 卷：454
• 期：1-2
• 页码：42-46
• 全文大小：352 K

文摘

Although oleate has been implicated in the regulation of phospholipase D (PLD) activity, the molecular identity of the oleate-stimulated PLD is still poorly understood. We now report that oleate selectively stimulates the enzymatic activity of PLD2 but not of PLD1, with an optimal concentration of 20 μM in vitro. Intriguingly, phosphatidylinositol 4,5-bisphosphate (PIP₂) synergistically stimulates the oleate-dependent PLD2 activity with an optimal concentration of 2.5 μM. These results provide the first evidence that oleate is a PLD2-specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP₂.