Central HIV-1 Tat exposure elevates anxiety and fear conditioned responses of male mice concurrent with altered mu-opioid receptor-mediated G-protein activation and β-arrestin 2 activity in the forebrain
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- 作者：Yun K. Hahna ; Jason J. Parisb ; Aron H. Lichtmanb ; c ; Kurt F. Hausera ; b ; c ; Laura J. Sim-Selleyb ; c ; Dana E. Selleyb ; c ; Pamela E. Knappa ; b ; c ; pamela.knapp@vcuhealth.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：Amyg ; Amygdala ; β-Arr2 ; β-arrestin-2 ; BSA ; bovine serum albumin ; CNS ; central nervous system ; Co-IP ; co-immunoprecipitation ; CPu ; caudate&ndash ; putamen ; [D-Ala2 ; N-MePhe4 ; Gly-ol]-enkephalin ; DAMGO ; DOX ; doxycycline ; EC50 ; ligand concentration causing half-maximal effect ; Emax ; maximal effect at full receptor occupancy ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; GDP ; guanosine diphosphate ; GFAP ; glial fibrillary acidic protein ; GPCR ; G-protein coupled receptor ; GRK2 ; G-protein receptor kin
- 刊名：Neurobiology of Disease
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：92
- 期：part_PB
- 页码：124-136
- 全文大小：1295 K
文摘
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Opiate-HIV co-exposure enhances CNS morphological and behavioral deficits.
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Such interaction may occur if HIV/HIV-1 Tat alters μ-opioid receptor (MOR) function.
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Tat altered morphine/DAMGO-stimulated G-protein activation by the MOR.
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Tat expression in vivo enhanced fear and anxiety behaviors without motor changes.
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Tat enhanced β-arrestin-2 binding to MOR specifically in amygdala, which may affect fear behavior.