- 作者：Gulden Avci M.D.&lowast ; ; ; drguldenavci@hotmail.com ; Huseyin Kadioglu M.D.&dagger ; ; Ahmet O. Sehirli M.D.&Dagger ; ; Suleyman Bozkurt M.D.&dagger ; ; Oguz Guclu M.D.§ ; ; Emrah Arslan M.D.&lowast ; ; Sedit K. Muratli M.D.¶ ;
- 关键词：ischemia-reperfusion injury ; skeletal muscle ; curcumin ; reactive oxygen species
- 刊名：Journal of Surgical Research
- 出版年：2012
- 出版时间：January 2012
- 年：2012
- 卷：172
- 期：1
- 页码：e39-e46
- 全文大小：368 K
Background
Curcumin has been shown to decrease ischemia-reperfusion (I/R) injury in kidney or brain tissues. In this study, the effects of curcumin were evaluated in skeletal muscle during I/R injury.Materials and Methods
Hind limb ischemia was induced by clamping the common femoral artery and vein. After 4 h ischemia, the clamp of the femoral vessels of animals was taken off and the animal underwent 2 h reperfusion. We measured plasma concentrations of interleukin-1¦Â (IL-1¦Â) and tumor necrosis factor-¦Á (TNF-¦Á) using enzyme-linked immunosorbent assay (ELISA). The right gastrocnemius muscle was harvested and immediately stored at ?0¡ãC for the assessment of superoxide dismutase (SOD), catalase (CAT) activities, and measurement of glutathione (GSH), malondialdehyde (MDA), and protein oxidation (PO) levels. Curcumin (100 mg/kg), ¦Á-tocopherol, and normal saline (10 mL /kg1) were administered intraperitoneally 1 h prior reperfusion.
Results
Plasma TNF-¦Á or IL-1¦Â levels increased significantly in I/R group. The plasma levels of these proinflammatory cytokines were reduced in curcumin group. Muscle tissues of I/R groups revealed significantly higher antioxidant enzyme (superoxide dismutase, glutathione peroxidase, catalase) activities, and increased levels of malondialdehyde, nitric oxide, and protein carbonyl content compared with the SHAM group. Levels of these parameters in muscle revealed significant reductions in the I/R + curcumin group compared witho the I/R group. Curcumin has more potent antioxidant activity than vitamin E in the skeletal muscle I/R.
Conclusion
In this study, protective effects of curcumin against skeletal muscle ischemia-reperfusion injury have been revealed. We underscore the necessity of human studies with curcumin that would be hypothetically beneficial preventing skeletal muscle I/R injury.