• 作者：Alessia Gallo^a ; Shyh-Ing Jang^a ; Hwei Ling Ong^b ; Paola Perez^a ; Mayank Tandon^a ; Indu Ambudkar^b ; Gabor Illei^a ; Ilias Alevizos^a ; ^{alevizosi@nidcr.nih.gov" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sjö ; gren's syndrome ; microRNA ; Exosomes ; STIM1 ; Salivary gland dysfunction
• 刊名：EBioMedicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：10
• 期：Complete
• 页码：216-226
• 全文大小：2384 K

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Sjögren's syndrome is an autoimmune disease characterized by dysfunction and inflammation of the salivary and lacrimal glands

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The pathogenesis of Sjögren's syndrome has not been elucidated, but the role of viruses has been suggested.

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Ebv-miR-BART13-3p downregulates STIM1 affecting the calcium influx mechanisms that regulate salivary gland function.

In this study we report that the EBV-specific microRNA ebv-miR-BART13-3p, that is significantly elevated in salivary glands of primary Sjögren's syndrome patients, targets stromal interacting molecule 1 (STIM1), a primary regulator of the store-operated Ca^2 + entry pathway that is essential for salivary gland function. This interaction affects the intracellular Ca^2 + entry and subsequently the Ca^2 +-dependent activation of NFAT. Ebv-miR-BART13-3p is present in both B cells and salivary epithelial cells, even though EBV infects B cells and not salivary epithelial cells.

We demonstrate here that ebv-miR-BART13-3p can be transferred from B cells to salivary epithelial cells through exosomes, recapitulating the functional effects on calcium signaling.