Comparative study on fatty acid metabolism of early stages of two crustacean species: Artemia sp. metanauplii and Grapsus adscensionis zoeae, as live prey for marine animals

详细信息    查看全文

• 作者：Diana B. Reis^a ; ^b ; ^c ; ^{dfbreis@ualg.pt} ; ^{diana_b_reis@hotmai.com} ; Nieves G. Acosta^a ; Eduardo Almansa^c ; Juan C. Navarro^d ; Douglas R. Tocher^e ; José ; P. Andrade^b ; Antó ; nio V. Sykes^b ; Covadonga Rodrí ; guez^a
• 关键词：Artemia sp. metanauplii ; Grapsus adscensionis zoeae ; Lipid metabolism ; Marine larvae ; Unsaturated fatty acids
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：204
• 期：Complete
• 页码：53-60
• 全文大小：244 K
• 卷排序：204

文摘

The present study compared the lipid composition and in vivo capability of Artemia sp. metanauplii (the main live prey used in aquaculture) and Grapsus adscensionis zoeae (as a wild zooplankton model) to metabolise unsaturated fatty acids. The two species were incubated in vivo with 0.3 μM of individual [1-14C]fatty acids (FA) including 18:1n‐9, 18:2n‐6, 18:3n‐3, 20:4n‐6 (ARA), 20:5n‐3 (EPA) and 22:6n‐3 (DHA) bound to bovine serum albumin (BSA). Compared to metanauplii, zoeae contained twice the content of polar lipids (PL) and eight-fold the content of long-chain polyunsaturated fatty acids (LC-PUFA). Artemia sp. metanauplii showed increased short chain fatty acid de novo synthesis from beta-oxidation of [1-14C]LC-PUFA, preferentially DHA. Of the LC-PUFA, DHA showed the highest esterification rate into Artemia sp. triacylglycerols. In contrast, in Grapsus zoeae [1-14C]DHA displayed the highest transformation rate into longer chain-length FAs and was preferentially esterified into PL. EPA and ARA, tended to be more easily incorporated and/or retained than DHA in Artemia sp. Moreover, both EPA and ARA were preferentially esterified into Artemia PL, which theoretically would favour their bioavailability to the larvae. In addition to the inherent better nutritional value of Grapsus zoeae due to their intrinsic lipid composition, the changes taking place after the lipid incorporation, point at two distinct models of lipid metabolism that indicate zoeae as a more suitable prey than Artemia sp. for the feeding of marine animals.