Confirming Sterility of an Autoclaved Infected Femoral Component for Use in an Articulated Antibiotic Knee Spacer: A Pilot Study

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• 作者：Steven T. Lyons ; MD^a ; ; Coy A. Wright ; MD^a ; Christina N. Krute ; BS^b ; Frances E. Rivera ; MS^b ; Ronan K. Carroll ; PhD^b ; Lindsey N. Shaw ; PhD^b
• 关键词：antibiotic spacer ; infected total knee arthroplasty ; biofilm ; autoclave ; articulating knee spacer ; two-stage revision
• 刊名：The Journal of Arthroplasty
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：31
• 期：1
• 页码：245-249
• 全文大小：530 K

文摘

Antibiotic spacer designs have proven effective at eradicating infection during a two-stage revision arthroplasty. Temporary reuse of the steam-sterilized femoral component and a new all poly tibia component has been described as an effective articulating antibiotic spacer, but sterility concerns persist. Six explanted cobalt chrome femurs from patients with grossly infected TKA's and six stock femurs inoculated with different bacterial species were confirmed to be bacteria-free after autoclaving under a standard gravity-displacement cycle. The effect of steam sterilization on cobalt chrome fragments contaminated with MRSA biofilm was analyzed microscopically to quantify remaining biofilm. The autoclave significantly reduced the biofilm burden on the cobalt chrome fragments. This study confirmed sterility of the femur after a standard gravity–displacement cycle (132 °C, 27 PSIG, 10 minutes).