Crystal Structures of the uL3 Mutant Ribosome: Illustration of the Importance of Ribosomal Proteins for Translation Efficiency
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- 作者：Justine Mailliot1 ; mailliot@igbmc.fr" class="auth_mail" title="E-mail the corresponding author ; Nicolas Garreau de Loubresse1 ; 4 ; nicolas.garreau@wyss.harvard.edu" class="auth_mail" title="E-mail the corresponding author ; Gulnara Yusupova1 ; gula@igbmc.fr" class="auth_mail" title="E-mail the corresponding author ; Arturas Meskauskas2 ; 3 ; arturas.meskauskas@gf.vu.lt" class="auth_mail" title="E-mail the corresponding author ; Jonathan D. Dinman2 ; dinman@umd.edu" class="auth_mail" title="E-mail the corresponding author ; Marat Yusupov1 ; marat@igbmc.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：rRNA ; ribosomal RNA ; tRNA ; transfer RNA ; PTC ; peptidyl-transferase center ; LSU ; large subunit ; W finger ; tryptophan finger ; aa-tRNA ; aminoacyl-tRNA ; pep-tRNA ; peptidyl-tRNA ; DTT ; dithiothreitol
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：22 May 2016
- 年：2016
- 卷：428
- 期：10
- 页码：2195-2202
- 全文大小：1123 K
文摘
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Ribosomal protein uL3 mutation W255C affects the regulation of protein synthesis.
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The structure of the mutant ribosome shows reorganization of the PTC A-site side.
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The mutant + anisomycin structure suggests that antibiotic and tRNA may bind together.
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Our data depict the importance of ribosomal proteins for translation efficiency.