Crystal structure of the 80S yeast ribosome
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- 作者：Lasse Jenner1 ; 2 ; 3 ; 4 ; Sergey Melnikov1 ; 2 ; 3 ; 4 ; Nicolas Garreau de Loubresse1 ; 2 ; 3 ; 4 ; Adam Ben-Shem1 ; 2 ; 3 ; 4 ; Madina Iskakova1 ; 2 ; 3 ; 4 ; Alexandre Urzhumtsev1 ; 2 ; 3 ; 4 ; 5 ; Arturas Meskauskas6 ; 7 ; Jonathan Dinman7 ; Gulnara Yusupova1 ; 2 ; 3 ; 4 ; Marat Yusupov1 ; 2 ; 3 ; 4 ; marat@igbmc.u-strasbg.fr
- 刊名：Current Opinion in Structural Biology
- 出版年：2012
- 出版时间：December, 2012
- 年：2012
- 卷：22
- 期：6
- 页码：759-767
- 全文大小：2968 K
文摘
The first X-ray structure of the eukaryotic ribosome at 3.0 ? resolution was determined using ribosomes isolated and crystallized from the yeast Saccharomyces cerevisiae (Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M: The structure of the eukaryotic ribosome at 3.0 A resolution. Science 2011, 334:1524-1529). This accomplishment was possible due to progress in yeast ribosome biochemistry as well as recent advances in crystallographic methods developed for structure determination of prokaryotic ribosomes isolated from Thermus thermophilus and Escherichia coli.
In this review we will focus on the development of isolation procedures that allowed structure determination (both cryo-EM and X-ray crystallography) to be successful for the yeast S. cerevisiae. Additionally we will introduce a new nomenclature that facilitates comparison of ribosomes from different species and kingdoms of life. Finally we will discuss the impact of the yeast 80S ribosome crystal structure on perspectives for future investigations.