Changes in nitric oxide/hydrogen peroxide content and cell cycle progression: Study with synchronized cultures of green alga Chlamydomonas reinhardtii

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• 作者：Wojciech Pokora^a ; ^{pokora@ug.edu.pl} ; Anna Aksmann^a ; Agnieszka Baścik-Remisiewicz^a ; Agnieszka Dettlaff-Pokora^b ; Max Rykaczewski^a ; Magdalena Gappa^a ; Zbigniew Tukaj^a
• 关键词：ABS/RC ; the amount of energy absorbed by single reaction center of photosystem II ; APX ; ascorbate peroxidase ; AsA ; ascorbic acid ; CAT ; catalase ; CYC ; cyclin ; CDK ; cyclin-dependent kinase ; Cu/Zn-SOD ; copper-zinc superoxide dismutase ; DI0/RC ; nonphotochemical energy dissipation by single reaction center of photosystem II ; DMSO ; dimethylsulfoxide ; DTT ; DL-dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; ET0/RC ; the amount of energy used for electron transport by single reaction center of phot
• 刊名：Journal of Plant Physiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：208
• 期：Complete
• 页码：84-93
• 全文大小：1686 K
• 卷排序：208

文摘

The present study aimed to evaluate the possible relationship between the changes in hydrogen peroxide (H2O2) and nitric oxide (NO) content and the course of growth and reproductive processes of the cell cycle of Chlamydomonas reinhardtii. The peak of H2O2 observed at the beginning of the cell cycle was found to originate from Fe-SOD and Mn-SODchl. activity and result from the alternation in the photosynthetic processes caused by the dark-to-light transition of daughter cells. A rapid increase in NO concentration, observed before the light-to-dark cell transition, originated from NR and NIR activity and was followed by a photosynthesis-independent, Mn-SODchl.-mediated increases in H2O2 production. This H2O2 peak overlapped the beginning of Chlamydomonas cell division, which was indicated by a profile of CYCs and CDKs characteristic of cells’ passage through the G1/S and S/M checkpoints. Taken together, our results show that there is a clear relationship between the course of the Chlamydomonas cell cycle and typical changes in the H2O2/NO ratio, as well as changes in expression and activity of enzymes involved in generation and scavenging of these signaling molecules.