γδT cells kill myeloma cells by sensing mevalonate metabolites and ICAM-1 molecules on cell surface
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- 作者：Ryo Uchida ; Eishi Ashihara ; Kiyoshi Sato ; Shinya Kimura ; Junya Kuroda ; Miki Takeuchi ; Eri Kawata ; Kyoko Taniguchi ; Masashi Okamoto ; Kazuho Shimura ; et al.
- 关键词：Immunotherapy ; Multiple myeloma ; γ ; δ ; T cells ; ICAM-1 ; Bisphosphonate
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 出版时间：9 March 2007
- 年：2007
- 卷：354
- 期：2
- 页码：613-618
- 全文大小：366 K
文摘
We evaluated the mechanism of recognition of myeloma cells by γδT cells. The expanded γδT cells killed RPMI8226 and U266 myeloma cells in a γδT-cell dose-dependent manner. Pretreatment of myeloma cells with zoledronic acid or mevastatin showed that γδT cells kill myeloma cells by recognizing the mevalonate metabolites. The expression level of intercellular cell adhesion molecule-1 (ICAM-1) on myeloma cells correlates with the cytotoxicity by γδT cells. Pretreatment of RPMI8226 and U266 with an anti-ICAM-1 monoclonal antibody inhibited their cytolysis. Moreover, AMO-1 myeloma cells transfected with of human ICAM-1 cDNA were susceptible to γδT cells compared to parental AMO-1 cells. In conclusion, γδT cells recognize the mevalonate metabolites and ICAM-1 on myeloma cells.