A combination of a DNA-chimera siRNA against PLK-1 and zoledronic acid suppresses the growth of malignant mesothelioma cells in vitro
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- 作者：Eri Kawata ; Eishi Ashihara ; Yoko Nakagawa ; Takahiro Kiuchi ; Mai Ogura ; Hisayuku Yao ; Kazuki Sakai ; Ruriko Tanaka ; Rina Nagao ; Asumi Yokota ; Miki Takeuchi ; Shinya Kimura ; Hideyo Hirai ; Taira Maekawa
- 关键词：PLK-1 ; RNA interference ; DNA-chimeric siRNA ; Bisphosphonate ; Malignant mesothelioma
- 刊名：Cancer Letters
- 出版年：2010
- 出版时间：28 August 2010
- 年：2010
- 卷：294
- 期：2
- 页码：245-253
- 全文大小：1409 K
文摘
Although novel agents effective against malignant mesothelioma (MM) have been developed, the prognosis of patients with MM is still poor. We generated a DNA-chimeric siRNA against polo-like kinase-1 (PLK-1), which was more stable in human serum than the non-chimeric siRNA. The chimeric PLK-1 siRNA inhibited MM cell proliferation through the induction of apoptosis. Next, we investigated the effects of zoledronic acid (ZOL) on MM cells, and found that ZOL also induced apoptosis in MM cells. Furthermore, ZOL augmented the inhibitory effects of the PLK-1 siRNA. In conclusion, combining a PLK-1 siRNA with ZOL treatment is an attractive strategy against MM.