High oxidative stress adversely affects NFκB mediated induction of inducible nitric oxide synthase in human neutrophils: Implications in chronic myeloid leukemia
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- 作者：Abhishek Kumar Singha ; Deepika Awasthia ; Megha Dubeya ; Sheela Nagarkotia ; Ashutosh Kumarb ; Tulika Chandrac ; Manoj Kumar Barthwala ; Anil Kumar Tripathid ; Madhu Dikshita ; madhu_dikshit@cdri.res.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Inducible nitric oxide synthase ; Nitric oxide ; NFκB ; Reactive oxygen species ; S-glutathionylation
- 刊名：Nitric Oxide
- 出版年：2016
- 出版时间：31 August 2016
- 年：2016
- 卷：58
- 期：Complete
- 页码：28-41
- 全文大小：2257 K
文摘
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Diamide or PMA induced enhanced oxidative stress attenuate basal as well as LPS/cytokines induced iNOS/NO generation.
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High ROS in PMNs of CML subjects linked with reduced iNOS/NO, and were unresponsive to LPS/cytokines mediated induction.
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S-Glutathionylation of NFκB under excessive ROS limits its binding to iNOS promoter.
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DTT reverses NFκB S-glutathionylation, improves DNA binding activity and enhanced iNOS expression/NO generation.
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Enhanced NOX2, mitochondrial ROS and reduced GSH/Grxs regulate NFκB S-glutathionylation in CML.