Cellular delivery of shRNA using aptamer-conjugated PLL-alkyl-PEI nanoparticles
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- 作者：Saeedeh Askariana ; askarians891@mums.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Khalil Abnousb ; abnouskh@mums.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Sahar Taghavib ; taghaviS881@mums.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Reza Kazemi Oskueea ; c ; oskueekr@mums.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Mohammad Ramezanib ; d ; ramezanim@mums.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aptamer ; Bcl-XL shRNA ; Gene delivery ; Non-viral vector ; Poly(l-lysine) ; Polyethylenimine
- 刊名：Colloids and Surfaces B: Biointerfaces
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：136
- 期：Complete
- 页码：355-364
- 全文大小：2286 K
文摘
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Two nanoparticles were synthesized with different combination of PLL and PEI polycations.
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Modified polymers showed better transfection efficiency and less cytotoxicity in compare to unmodified PEI.
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Targeted polycationic vectors were produced by conjugation of nucleoline aptamer to modified polymers.
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Aptamer-conjugated nanoparticles efficiently delivered shRNA-plasmid (against an anti-apoptotic gene) to lung cancer cell line (a549).
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Following down-regulation of the anti-apoptotic gene (bcl-xl), apoptosis induced in targeted cells (and not in control cell).