Mutational analysis of Kex2 recognition sites and a disulfide bond in tannase from Aspergillus oryzae

详细信息    查看全文

• 关键词：Tannase ; Kex2 recognition site ; Disulfide bond ; Aspergillus oryzae
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：1165-1169
• 全文大小：467 K
• 卷排序：482

文摘

We investigated the functional roles of the Kex2 recognition sites and the disulfide bond. There was no effects of the R311A/R316A variant on the activity and stability. The ΔKR variant exhibited higher activity compared to the wild-type. The activities of the C194A and C502A variants decreased considerably (<0.24% of the wild-type).