Development of a cleaning in place protocol and repetitive application of Escherichia coli homogenate on STREAMLINE™ Q XL

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• 作者：Magnus Asplund ; Mats Ramberg ; Bo-Lennart Johansson
• 关键词：Cleaning in place (CIP) protocol ; STREAMLINE™ ; Q XL ; Escherichia coli
• 刊名：Process Biochemistry
• 出版年：2000
• 出版时间：July 2000
• 年：2000
• 卷：35
• 期：10
• 页码：1111-1118
• 全文大小：984 K

文摘

An optimization of a cleaning in place (CIP) protocol has been performed for STREAMLINE™ Q XL separation medium using homogenised Escherichia coli as sample. The effectiveness of the different cleaning protocols was tested by analysing the content of fatty acids, phosphorus compounds and amino acids in STREAMLINE™ Q XL. Additionally, RAMAN and FT-IR measurements were used to verify the degree of contamination of separation medium. From the results of the optimization experiments, a cleaning procedure was suggested and subsequently tested with 50 repetitive applications. The study shows that of the different agents tested, 1 M NaOH is the most efficient cleaning solution and that long contact time (8 h) with 1 M NaOH is important. An additional cleaning step with 30 % 2-propanol (v/v) is also recommended for E. coli samples.