Determination of amphetamines in human whole blood by capillary electrophoresis with photodiode array detection

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• 作者：Boatto ; Gianpiero ; Faedda ; Maria Virginia ; Pau ; Amedeo ; Asproni ; Battistina ; Menconi ; Sonia ; et. al.
• 关键词：Amphetamines ; Human blood ; Capillary electrophoresis ; Photodiode array
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2002
• 出版时间：August 1, 2002
• 年：2002
• 卷：29
• 期：6
• 页码：1073-1080
• 全文大小：219 K

文摘

A capillary electrophoresis (CE) with photodiode array detection (DAD) method for the analysis of amphetamines in human whole blood samples is described. Amphetamines were applied to CE without any derivatization procedure and detected at 200 nm for a rapid and simple analysis. The UV-spectra are show in . Amphetamines were separated within 7 min through an uncoated fused-silica capillary (50 cm×50 μm ID) using a 100 mM phosphate buffer (pH 2.5). A simple and fast extraction method of amphetamines from human whole blood was developed using acetonitrile. Very clean extracts were obtained in one step. Whole blood drugs free samples were spiked with amphetamine standard solution of known concentration. Linear calibration plots were obtained over a large concentration range, with correlation coefficients higher than 0.998. Recoveries between 81 and 99 % were obtained. Limit of detection (LOD) was from 10 to 30 ng ml⁻¹ for most of amphetamines, except for MDMA, for which it was 80 ng ml⁻¹.