Cryo-SPIEM affinity grid method enables cryo-EM studies of many diluted specimens.

Different affinity layers can be generated through the protein-surface adsorption.

The affinity grid technique would assist studies of macromolecular intermediates.

Affinity grids will make cryo-EM a widely applicable structural biology technique.

Use of affinity grids would help develop cryo-EM into a high-throughput technique.