Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer

详细信息    查看全文

• 作者：Mingzhen Zhang^a ; ^b ; ^{mzhang21@gsu.edu" class="auth_mail" title="E-mail the corresponding author} ; Emilie Viennois^a ; ^b ; Meena Prasad^c ; ^d ; Yunchen Zhang^a ; ^b ; Lixin Wang^a ; ^b ; ^c ; Zhan Zhang^a ; ^b ; Moon Kwon Han^a ; ^b ; Bo Xiao^a ; ^b ; ^e ; Changlong Xu^a ; ^b ; ^f ; Shanthi Srinivasan^c ; ^d ; Didier Merlin^a ; ^b ; ^c
• 关键词：Edible ginger derived nanoparticles ; Inflammatory bowel disease ; Colitis-associated cancer ; Natural drug delivery system ; Therapy
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：101
• 期：Complete
• 页码：321-340
• 全文大小：7180 K

文摘

There is a clinical need for new, more effective treatments for chronic and debilitating inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis. In this study, we characterized a specific population of nanoparticles derived from edible ginger (GDNPs 2) and demonstrated their efficient colon targeting following oral administration. GDNPs 2 had an average size of ∼230 nm and exhibited a negative zeta potential. These nanoparticles contained high levels of lipids, a few proteins, ∼125 microRNAs (miRNAs), and large amounts of ginger bioactive constituents (6-gingerol and 6-shogaol). We also demonstrated that GDNPs 2 were mainly taken up by intestinal epithelial cells (IECs) and macrophages, and were nontoxic. Using different mouse colitis models, we showed that GDNPs 2 reduced acute colitis, enhanced intestinal repair, and prevented chronic colitis and colitis-associated cancer (CAC). 2D-DIGE/MS analyses further identified molecular target candidates of GDNPs 2 involved in these mouse models. Oral administration of GDNPs 2 increased the survival and proliferation of IECs and reduced the pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β), and increased the anti-inflammatory cytokines (IL-10 and IL-22) in colitis models, suggesting that GDNPs 2 has the potential to attenuate damaging factors while promoting the healing effect. In conclusion, GDNPs 2, nanoparticles derived from edible ginger, represent a novel, natural delivery mechanism for improving IBD prevention and treatment with an added benefit of overcoming limitations such as potential toxicity and limited production scale that are common with synthetic nanoparticles.