A novel human KRAB-containing zinc-finger gene ZNF446 inhibits transcriptional activities of SRE and AP-1

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• 作者：Liu ; Fang ; Zhu ; Chuanbing ; Xiao ; Jing ; Wang ; Yuequn ; Tang ; Wenxian ; Yuan ; Wuzhou ; Zhao ; Yulian ; et. al.
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2005
• 出版时间：July 22, 2005
• 年：2005
• 卷：333
• 期：1
• 页码：5-13
• 全文大小：1.04 M

文摘

Kruppel-related zinc-finger proteins constitute the largest individual family of transcription factors in mammals [C. Looman, L. Hellman, M. Abrink, A novel Kruppel-associated box identified in a panel of mammalian zinc-finger proteins, Mammalian Genome 15 (1) (2004) 35–40. [1]]. Here we identified and characterized a novel zinc-finger gene named ZNF446. The predicted protein contains a KRAB and three C₂H₂ zinc fingers. Northern blot analysis shows that ZNF446 is expressed in a variety of human adult tissues with the highest expression level in muscle. ZNF446 is a transcription repressor when fused to GAL4 DNA-binding domain and co-transfected with VP-16. Overexpression of ZNF446 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1, in which the KRAB motif represents the basal transcriptional repressive activity, suggesting that the ZNF446 protein may act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.