CagA subtyping in Helicobacter pylori isolates from gastric cancer patients in an ethnic Kashmiri population

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• 作者：Qurteeba Qadri ; Dil Afroze ; Roohi Rasool ; G.M. Gulzar ; Sameer Naqash ; Mushtaq A. Siddiqi ; Zafar A. Shah
• 关键词：Helicobacter pylori ; CagA protein ; Gastric cancer ; EPIYA motifs
• 刊名：Microbial Pathogenesis
• 出版年：January, 2014
• 年：2014
• 卷：66
• 期：Complete
• 页码：40-43
• 全文大小：247 K

文摘

The association between gastric cancer and Helicobacter pylori has been well established. Among H.聽pylori virulence genes the most important determinant is the cytotoxin associated antigen gene (cagA) which is characterized by the presence of repeated EPIYA motifs at the C terminus of the protein. From the alignment and number of these EPIYA motifs, two major types of CagA protein have been identified.

Aims

The aim of this study was to classify the CagA into eastern or western type and to determine the number and type of motifs present.

Methods

The CagA subtyping was done by PCR and multiplex PCR for eastern/western classification and determination of EPIYA motifs respectively.

Results

All the isolates studied were of the western type, with 70% of the isolates having more than one EPIYA-C motifs. No statistically significant association was found between the presence of CagA and more than one EPIYA-C motifs with the clinical outcome (differentiation status of the tumour).