KAP1-independent transcriptional repression of SCAN-KRAB-containing zinc finger proteins

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• 作者：Yasuhide Itokawa ; Toshihide Yanagawa ; Hisashi Yamakawa ; Naoko Watanabe ; Hisashi Koga ; Takahiro Nagase
• 关键词：Transcriptional repression ; KRAB-ZNF ; SCAN domain ; KAP1 ; HaloTag
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：30 October 2009
• 年：2009
• 卷：388
• 期：4
• 页码：689-694
• 全文大小：320 K

文摘

The Krüppel-associated box-containing zinc finger gene family (KRAB-ZNF) is one of the largest gene families of transcriptional factors in the human genome. Although the functions of most of these genes remain to be determined, it is known that KRAB-mediated transcriptional repression requires a direct interaction with the KAP1 co-repressor. By mammalian one- or two-hybrid experiments in HEK293 cells, we compared transcriptional repression activities of 61 human KRAB-ZNFs. The results showed that six SCAN-KRAB-containing ZNFs are KAP1-independent transcriptional repressors whose SCAN-KRAB domain is unable to associate with KAP1 despite retaining transcriptional repression activity. Transcriptional repression activities of the SCAN-KRAB domain of KAP1-independent KRAB-ZNFs are not influenced by depletion of endogenous KAP1 levels by small interfering RNA. Although the mechanism by which KAP1-independent KRAB-ZNFs repress transcriptional activity remains to be elucidated, it appears that there may be a pathway for transcriptional repression that does not involve KAP1. These results provide new insight into the functions of the members of the KRAB-ZNF family.