Simultaneous enantioseparation and purity determination of chiral switches of amlodipine and atenolol by liquid chromatography
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- 作者：Valliappan Kannappan ; vallikonnect@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Sai Sandeep Mannemala reddysince1989@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Amlodipine ; Atenolol ; Enantioseparation ; Chiral switches ; Enantiopurity assay ; Liquid chromatography
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：20 February 2016
- 年：2016
- 卷：120
- 期：Complete
- 页码：221-227
- 全文大小：1174 K
文摘
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First report describing the simultaneous determination of amlodipine and atenolol enantiomers by direct chiral HPLC.
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Systematic assessment of effects of individual components of mobile phase and their interaction on enantioseparation was evaluated using Box–Behnken design.
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Robustness was tested using Plackett–Burman design of experiments and Robust domains were plotted using contour profiling.
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Applicability of method was tested by analyzing three different commercially available enantiopure formulations.
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Obtained results demonstrated the suitability of proposed method in routine enantiopurity control of chiral switches of amlodipine and atenolol.