First report describing the simultaneous determination of amlodipine and atenolol enantiomers by direct chiral HPLC.
Systematic assessment of effects of individual components of mobile phase and their interaction on enantioseparation was evaluated using Box–Behnken design.
Robustness was tested using Plackett–Burman design of experiments and Robust domains were plotted using contour profiling.
Applicability of method was tested by analyzing three different commercially available enantiopure formulations.
Obtained results demonstrated the suitability of proposed method in routine enantiopurity control of chiral switches of amlodipine and atenolol.