Quercus infectoria galls possess antioxidant activity and abrogates oxidative stress-induced functional alterations in murine macrophages

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• 作者：Gurpreet Kaur ; Mohammad Athar ; M. Sarwar Alam
• 关键词：Quercus infectoria ; Galls ; Antioxidant ; Macrophages ; Phagocytic functions ; Oxidative stress ; tBOOH
• 刊名：Chemico-Biological Interactions
• 出版年：2008
• 出版时间：15 February 2008
• 年：2008
• 卷：171
• 期：3
• 页码：272-282
• 全文大小：720 K

文摘

The present study reports the antioxidant activity of ethanolic extract of Quercus infectoria galls. The antioxidant potency of galls was investigated employing several established in vitro model systems. Their protective efficacy on oxidative modulation of murine macrophages was also explored. Gall extract was found to contain a large amount of polyphenols and possess a potent reducing power. HPTLC analysis of the extract suggested it to contain 19.925 % tannic acid (TA) and 8.75 % gallic acid (GA). The extract potently scavenged free radicals including DPPH (IC₅₀0.5 μg/ml), ABTS (IC₅₀1 μg/ml), hydrogen peroxide (H₂O₂) (IC₅₀2.6 μg/ml) and hydroxyl (OH) radicals (IC₅₀6 μg/ml). Gall extract also chelated metal ions and inhibited Fe³⁺–ascorbate-induced oxidation of protein and peroxidation of lipids. Exposure of rat peritoneal macrophages to tertiary butyl hydroperoxide (tBOOH) induced oxidative stress in them and altered their phagocytic functions. These macrophages showed elevated secretion of lysosomal hydrolases, and attenuated phagocytosis and respiratory burst. Activity of macrophage mannose receptor (MR) also diminished following oxidant exposure. Pretreatment of macrophages with gall extract preserved antioxidant armory near to control values and significantly protected against all the investigated functional mutilations. MTT assay revealed gall extract to enhance percent survival of tBOOH exposed macrophages. These results indicate that Q. infectoria galls possess potent antioxidant activity, when tested both in chemical as well as biological models.