Cumene hydroperoxide debilitates macrophage physiology by inducing oxidative stress: Possible protection by α-tocopherol

详细信息    查看全文

• 作者：Gurpreet Kaur ; M. Sarwar Alam ; Mohammad Athar
• 关键词：Macrophages ; Cumene hydroperoxide ; α ; -Tocopherol ; Phagocytic functions ; Mannose receptors
• 刊名：Chemico-Biological Interactions
• 出版年：2009
• 出版时间：15 May 2009
• 年：2009
• 卷：179
• 期：2-3
• 页码：94-102
• 全文大小：969 K

文摘

Macrophages, the major phagocytes of body, are largely dependent on membrane for their apposite functioning. Cum-OOH, a catalyst used in chemical and pharmaceutical industry, is a peroxidative agent, which may induce oxidative stress in macrophages hampering the integrity of their membrane. α-Tocopherol is known to protect the membrane from oxidative modulation and preserve its integrity. In the present study, we investigated the effect of Cum-OOH on physiology of macrophages and evaluated the protective effect of α-tocopherol against Cum-OOH-induced functional impairment. An in vitro exposure to 10–200 μM Cum-OOH altered redox balance of murine peritoneal macrophages and led to a severe physiological impairment. It markedly augmented the release of proinflammatory cytokines (tumor necrosis factor-α, interleukin-1β and prostaglandin E₂), lipopolysaccharide primed nitric oxide release and inducible nitric oxide synthase expression, and lysosomal hydrolases secretion. It mitigated respiratory burst and phagocytosis and intracellular killing of yeast (Saccharomyces cereviseae). Mannose receptor, a major macrophage phagocytic receptor (also implicated in S. cereviseae phagocytosis), exhibited a hampered recycling with its number being reduced to about 54 % of the untreated, control cells following Cum-OOH exposure. A 24-h pretreatment of macrophages with 25 μM α-tocopherol preserved most of the assessed functions close to their corresponding control values. These data suggest that exposure to Cum-OOH may impair the physiology of immune cells such as macrophages and that supplementation with α-tocopherol can safeguard these cells against Cum-OOH toxicity.