Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides

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• 作者：Atsuko Shimoyama^a ; ^b ; Aki Fujisaka^b ; ^c ; Satoshi Obika^b ; ^{obika@phs.osaka-u.ac.jp}
• 关键词：Size exclusion chromatography ; Phosphorothioate oligonucleotides ; Nucleic acids therapeutics
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：20 March 2017
• 年：2017
• 卷：136
• 期：Complete
• 页码：55-65
• 全文大小：2707 K
• 卷排序：136

文摘

Phosphorotioate oligonucleotides are analyzed using size exclusion chromatography. They interact with column packing material and thereby they elute late and broadly. Single- and double-stranded phosphorothioate oligonucleotides are discriminated.