Structural basis of the substrate recognition of hydrazidase isolated from Microbacterium sp. strain HM58-2, which catalyzes acylhydrazide compounds as its sole carbon source

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• 作者：Tomonori Akiyama^a ; Misaki Ishii^a ; Atsushi Takuwa^b ; Ken-Ichi Oinuma^b ; Yasuyuki Sasaki^a ; Naoki Takaya^b ; Shunsuke Yajima^a ; ^{yshun@nodai.ac.jp}
• 关键词：Amidase signature family ; Microbacterium ; Protein structure ; Substrate specificity ; X-ray crystallography
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：1007-1012
• 全文大小：1562 K
• 卷排序：482

文摘

The crystal structure of hydrazidase complexed with a substrate is determined. A catalytic triad of Ser-cisSer-Lys in hydrazidase is revealed. His336 in a non-conserved region is a determinant for substrate specificity.