A pilot study of antioxidant potential of endophytic fungi from some Sudanese medicinal plants

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• 作者：Afra Khiralla¹ ; ² ; ³ ; Ietidal Mohamed³ ; Justinne Thomas⁴ ; Benoî ; t Mignard⁴ ; Rosella Spina¹ ; ² ; Sakina Yagi³ ; Dominique Laurain-Mattar¹ ; ² ; ^{dominique.mattar@univ-lorraine.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sudanese medicinal plants ; Endophytic fungi ; Antioxidant activity ; Total phenolic content
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：8
• 期：9
• 页码：701-704
• 全文大小：436 K

文摘

To evaluate the total phenolic content and total antioxidant capacity of ethyl acetate extracts of 21 endophytic fungi isolated from five Sudanese medicinal plants: Calotropis procera, Catharanthus roseus, Euphorbia prostrate, Vernonia amygdalina and Trigonella foenum-graecum.

Methods

Crude extracts of endophytic fungi and their host plants were tested by classical Folin–Ciocalteu colorimetric method to determine the total phenolic content, also total antioxidant capacity was estimated using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging in vitro method.

Results

Among the endophytes, endophytic fungus Aspergillus sp. from Trigonella foenum-graecum seeds demonstrated the highest both total phenolic content in term of gallic acid equivalent [(89.9 ± 7.1) mg GAE/g] and antioxidant activity for 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay [IC₅₀: (18.0 ± 0.1) 渭g/mL]. A high positive linear correlation (R² = 0.999 1) was found between total antioxidant capacity and total phenolic content of endophytic fungi isolated from Vernonia amygdalina.

Conclusions

The present study revealed that some endophytic fungi from the five Sudanese medicinal plants could be a potential source of novel natural antioxidant compounds.